QT-101: Label-free Quantitative Proteome Profiling

QT-101: Label-free Quantitative Proteome Profiling

  • Description
  • Additional Info

Description

This service package is for proteome profiling (identify up to 5,000 proteins per sample) from human/mouse tissue or cell line samples using label-free quantitation method. This service package is also for proteome profiling of samples from other species. Please contact us for a quote.

Price: $3,000/sample

Turnaround time: 15 to 20 business days

Service Description

  • Identification and quantification of the entire set of proteins (the proteome) expressed by a cell, tissue or organism as many as possible.
  • More than 4000 (QT-101) proteins can be identified at 1% FDR from one sample.
  • The profile is evaluated for integrity and variability using PROMICPATH.
  • Work-flow: Protein lysate preparation, fractionation (16 fractions), digestion, 16 LC-MS/MS 110 min runs, common pathway analysis, and result report.

Deliverables

  • A list of identified proteins with relative abundance
  • A list of all identified proteins with matched peptides sequences
  • A list of proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥ 2 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (200 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥200 μg of total protein recommended for optimal results)
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