QT-201: TMTsixplex-Labeling Quantitative Proteome Profiling

  • Description
  • Additional Info

Description

This service package is for quantitatively comparison of the proteomes (>5000 proteins if human or mouse origin samples) of 2-6 samples in one set of analysis using Thermo TMTsixplex™ Isobaric Label Reagent.

Price: $8,000-$10,000/set

Turnaround time: 20 to 25 business days.

Service Description

  • Identification and quantification of the entire set of proteins (the proteome) expressed by a cell, tissue or organism for up to 10 samples in one set of analysis simultaneously.
  • More than 5000 proteins can be identified at 1% FDR from one sample.
  • The profile is evaluated for integrity and variability using PROMICPATH.
  • Work-flow:  Protein lysate preparation, TMT-labeling, UHPLC fractionation, digestion, LC-MS/MS analysis, TMT quantitation, Pathway analysis, and result report.

Deliverables

  • A list of identified proteins with TMT-tag based relative abundance
  • A list of all identified proteins with matched peptides sequences
  • A list of proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥10 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (≥500 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥500 μg of total protein recommended for optimal results)
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