Cas9 mRNAs (N1-Me-Pseudo UTP)
PROMICFINDER Platform for Protein Identification and Quantification
The combination of high-performance liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) has emerged as a powerful and universal method for the global measurement of proteins. Unlike traditional western-blot or ELISA methods, MS-based technology can identify and quantify protein mixtures simultaneously on a large scale. For interested proteins without an available antibody, MS-based protein identification is the fastest and most reliable approach.
PROMICFINDER, developed at Poochon, is a mass spectrometry-based protein analysis technology capable of identifying and measuring rapidly (high throughput), broadly (thousands of proteins simultaneously), and deeply (high and low-abundance proteins).
The PROMICFINDER Platform is a highly sensitive, quantitative, and reproducible proteomic tool for the profiling of proteins (up to 3,000 different proteins) from a single gel band sample, IP sample, or un-fractioned cell/tissue lysate sample, by a 110 min LC-MS/MS run.
Standardized SOPs for sample preparation and LC-MS/MS analysis ensure the quality and reproducibility of the data. Results are reported in a standardized format ready for generating publishable data.
New Mouse Line Genotyping Analysis Setup
Overview:
After transfection, Cas9 mRNA (N1-Me-Pseudo UTP) is expressed in cells to produce Cas9 protein derived from Streptococcus pyogenes SF370 (CRISPR-associated protein 9). This protein is a component of the CRISPR genome-editing system. In the CRISPR system, when co-transfected with a guide RNA (gRNA) targeting a specific genomic locus, Cas9 protein cleaves the corresponding site of the target gene. This product is an in vitro-transcribed Cas9 mRNA that can be transiently transfected into cells for performing CRISPR/Cas9-mediated genome editing.
Composition:
CT039-01 (100 μg)
CT039-10 (500 μg)
CT039-100 (1 mg)
Product Name: Cas9 mRNA (N1-Me-Pseudo UTP)
mRNA Length: 4451 nt
Concentration: 2 mg/mL
Storage buffer: MilliQ water
Storage Temperature: -80°C to -65°C
Shipping Conditions: Shipped on dry ice
Applications:
This product is intended for use in CRISPR/Cas9-mediated genome editing guided by
specific gRNAs.
Notes:
This product is for research use only. It is not intended for use in human or veterinary
diagnosis or therapy.