FLuc mRNA (N1-Me-Pseudo UTP)
PROMICFINDER Platform for Protein Identification and Quantification
The combination of high-performance liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) has emerged as a powerful and universal method for the global measurement of proteins. Unlike traditional western-blot or ELISA methods, MS-based technology can identify and quantify protein mixtures simultaneously on a large scale. For interested proteins without an available antibody, MS-based protein identification is the fastest and most reliable approach.
PROMICFINDER, developed at Poochon, is a mass spectrometry-based protein analysis technology capable of identifying and measuring rapidly (high throughput), broadly (thousands of proteins simultaneously), and deeply (high and low-abundance proteins).
The PROMICFINDER Platform is a highly sensitive, quantitative, and reproducible proteomic tool for the profiling of proteins (up to 3,000 different proteins) from a single gel band sample, IP sample, or un-fractioned cell/tissue lysate sample, by a 110 min LC-MS/MS run.
Standardized SOPs for sample preparation and LC-MS/MS analysis ensure the quality and reproducibility of the data. Results are reported in a standardized format ready for generating publishable data.
New Mouse Line Genotyping Analysis Setup
Overview:
FLuc mRNA (N1-Me-Pseudo UTP) encodes firefly luciferase (FLuc). After transfection, it is expressed in cells to produce firefly luciferase, which was originally derived from the firefly Photinus pyralis. Luciferase catalyzes the oxidation of luciferin to oxyluciferin, emitting yellow-green bioluminescence with a wavelength of 550–570 nm. The light intensity is directly proportional to the amount of luciferase expressed. This product can be used as a positive control for mRNA transfection, or for verification of delivery systems and manufacturing processes.
Composition:
CT072-01 (100 μg)
CT072-10 (500 μg)
CT072-100 (1 mg)
Product Name: FLuc mRNA (N1-Me-Pseudo UTP)
mRNA Length: 1913 nt
Concentration: 1 mg/mL
Storage buffer: MilliQ water
Storage Temperature: -80°C to -65°C
Shipping Conditions: Shipped on dry ice
Applications:
1. Optimization of formulation or manufacturing process
2. Screening and validation of delivery vectors
3. Positive control
4. Verification of expression systems
Notes:
This product is for research use only. It is not intended for use in human or veterinary
diagnosis or therapy.