Protein Identification

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Identification of Proteins (Up to 2,000 Proteins) From a Single Gel Band or Gel Spot

Price: $250 per sample

Turnaround Time: 5 to 10 business days

• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report

Deliverables

• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request

Suitable Sample Type

• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)

Identification of Proteins (Up to 4,000 Proteins) From Any Biological Sample

Price: $500 per sample

Turnaround Time: 5 to 10 business days

Service Description

• Identification of proteins from mixture samples including cell lysates, tissue lysates, IP, pull-down, or other types of protein mixtures
• Up to 4,000 proteins identified from one sample
• Target protein detection limit is at ng level
• Workflow: Sample preparation, In-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report

Deliverables

• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Comparisons between and among samples using PSM# and MS1 peak area label-free based quantification method
• Raw data files, upon request

Suitable Sample Type

• Purified protein, in solution or dried
• Purified protein complex
• Protein mixture from pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches (> 1 μg is required; > 5 μg is recommended)
• Fresh frozen cell/tissue lysate, body fluids, or cell culture medium

PROMICFINDER Platform for Protein Identification and Quantification

The combination of high-performance liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) has emerged as a powerful and universal method for the global measurement of proteins. Unlike traditional western-blot or ELISA methods, MS-based technology can identify and quantify protein mixtures simultaneously on a large scale. For interested proteins without an available antibody, MS-based protein identification is the fastest and most reliable approach.

PROMICFINDER, developed at Poochon, is a mass spectrometry-based protein analysis technology capable of identifying and measuring rapidly (high throughput), broadly (thousands of proteins simultaneously), and deeply (high and low-abundance proteins).

The PROMICFINDER Platform is a highly sensitive, quantitative, and reproducible proteomic tool for the profiling of proteins (up to 3,000 different proteins) from a single gel band sample, IP sample, or un-fractioned cell/tissue lysate sample, by a 110 min LC-MS/MS run.

Standardized SOPs for sample preparation and LC-MS/MS analysis ensure the quality and reproducibility of the data. Results are reported in a standardized format ready for generating publishable data.