Whole PLasmid Sequencing
About
Nanopore sequencing is a unique, scalable technology that enables direct, real-time analysis of long DNA or RNA fragments. It works by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence.
About
Nanopore sequencing is a unique, scalable technology that enables direct, real-time analysis of long DNA or RNA fragments. It works by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence.
Nanopore sequencing is a unique, scalable technology that enables direct, real-time analysis of long DNA fragments. It works by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA sequence.
Using Poochon’s Whole Plasmid Sequencing Services, entire plasmids up to 30 kb can be sequenced for $15/sample and no primer is required! Sequence results, including a fully annotated plasmid map, are delivered within 24 hours. With various drop box locations around the DC/Baltimore area, Poochon offers convenient sample submission options with weekday pickup daily.
Nanopore DNA Sequencing (up to 30 kb) - Plasmid Samples
Turnaround Time: < 24 hours
Service Description:
Long read Plasmid DNA sequencing by Oxford Nanopore Technology
Deliverables:
DNA sequence in TXT and FASTA file format, plus a fully annotated plasmid map
Suitable Sample Type:
Purified plasmid DNA (size 1.0 kb – 30 kb), in pure H2O, average concentration ≥ 50 ng/µl, OD260/280 = 1.8-1.95, OD260/230 = 2.0-2.2
• For 1 kb – 3 kb plasmid, 10 µl of sample per sequencing reaction with a concentration ≥ 10 ng/µl DNA is required
• For 3 kb – 15 kb plasmid, 10 µl of sample per sequencing reaction with a concentration ≥ 25 ng/µl DNA is required
• For 15 kb – 30 kb plasmid, 10 µl of sample per sequencing reaction with a concentration ≥ 50 ng/µl DNA is required
• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report
• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request
• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)
Nanopore DNA Sequencing (up to 30 kb) - Purified PCR Amplicon Samples
Turnaround Time: < 24 hours
Service Description:
Long read Amplicon DNA sequencing by Oxford Nanopore Technology
Deliverables:
DNA sequence in TXT and FASTA file format
Suitable Sample Type:
Purified PCR Amplicon DNA (size 0.5 kb – 30 kb), in pure H2O, average concentration ≥ 25 ng/µl, OD260/280 = 1.8-1.95, OD260/230 = 2.0-2.2
• For 0.5 – 3 kb Amplicon, 10 µl of sample per sequencing reaction with a concentration ≥ 5 ng/µl DNA is required
• For 3 – 15 kb Amplicon, 10 µl of sample per sequencing reaction with a concentration ≥ 25 ng/µl DNA is required
• For 15 – 30 kb Amplicon, 10 µl of sample per sequencing reaction with a concentration ≥ 50 ng/µl DNA is required
• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report
• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request
• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)
Nanopore DNA Sequencing (up to 30 kb) - Bacteria Colony Samples
Turnaround Time: 48 hours
Service Description:
Colony incubation, plasmid MiniPrep, long read DNA sequencing by Oxford Nanopore Technology
Deliverables:
DNA sequence in TXT and FASTA file format, plus a fully annotated plasmid map
Suitable Sample Type:
Colony suspension, liquid cultures, glycerol stock, or agar plate (plasmid size 2 kb – 30 kb)
Please be sure to indicate the correct antibiotic when placing the order
No sequencing primer required!
• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report
• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request
• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)
Nanopore DNA Sequencing (up to 200 kb)
Turnaround Time: Varies, typically 2 to 3 business days
Service Description:
Long read DNA sequencing by Oxford Nanopore Technology
Deliverables:
DNA sequence in TXT and FASTA file format, plus a fully annotated plasmid map
Suitable Sample Type:
Plasmid DNA or purified PCR product (30 kb – 200 kb)
Please submit sequencing samples in 8-strip PCR tubes
No sequencing primer required!
• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report
• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request
• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)
+ N104: Standard Nanopore Whole Plasmid Sequencing Tubes
Standard Nanopore DNA Sequencing (up to 30 Kb)
Turnaround Time: 24 hours
Service Description:
Long read DNA sequencing by Oxford Nanopore Technology
Deliverables:
DNA sequence in TXT and FASTA file format, plus a fully annotated plasmid map
Suitable Sample Type:
Minimum quantity = 200
• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report
• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request
• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)
Sample Preparation
Sample Type
Plasmid DNA or ≥ 2 kb PCR
Size
Up to 30 kb Plasmid
OD Ratio
Minimum Volume
5 μl, 10 μl preferred
Concentration
100-200 ng/μl
Features & Benefits
- Starting at $15 per sample
- No sequencing primers required
- Free local sample pick-up available
- 24-Hour turnaround time
- No minimum number of samples required
- Automated data analysis