Intact Protein Analysis

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Intact Protein Molecular Mass Measurement of Purified Protein Samples Using Intact Protein Mass Spectrometry Analysis Technology

Price: $450 per sample

Turnaround Time: 5 to 10 business days

• Molecular weight determination of purified protein samples with an expected molecular mass less than 160 kDa, a mass accuracy < 20 ppm, and a mass range from 40 to 8,000 m/z
• Workflow: Sample preparation, intact protein mode LC-MS analysis, data analysis, and results report

Deliverables

• Protein Analysis Report
• Full MS spectra of intact protein peak from Liquid Chromatography-Mass Spectrometry
• Deconvoluted spectrum range from 10 kDa to 160 kDa showing the purity and molecular weight
• Data figures
• Raw data files, upon request

Suitable Sample Type

• Purified protein sample, in solution or dried (≥ 20 µg per sample recommended for optimal results, purity > 90%)

Protein Molecular Mass Measurement

Intact protein analysis provides information on the accurate mass of the protein and the relative abundance of its isoforms and modifications. It is an invaluable approach for the analysis of biologics throughout manufacturing to assess purity and heterogeneity characteristics for lot release purposes.

Poochon’s intact protein analysis services are designed for intact protein mass measurement to confirm the sequence of a purified protein and the relative abundance of its isoforms, for example a modification with different glycans. Intact protein characterization is performed on samples in solution using a combination of liquid chromatography (LC) and electrospray ionization mass spectrometry (ESI-MS). Purified proteins of molecular weight up to 160 kDa at a minimum amount of 1 µg can be analyzed successfully.

Examples using Intact Protein Analysis:

Analysis of glycan forms and molecular weight of mAbs
Analysis of molecular weight of light and heavy chains of mAbs
Analysis of drug-to-antibody ratio of antibody-drug conjugates