Protein Identification

PROMICFINDER Platform for Protein Identification and Quantification

The combination of high-performance liquid chromatography and high-resolution tandem mass spectrometry (LC-MS/MS) has emerged as a powerful and universal method for the global measurement of proteins. Unlike traditional western-blot or ELISA methods, MS-based technology can identify and quantify protein mixtures simultaneously on a large scale. For interested proteins without an available antibody, MS-based protein identification is the fastest and most reliable approach.

+ P101: Protein Identification of Gel Bands or Gel Spots
Identification of Proteins (Up to 2,000 Proteins) From a Single Gel Band or Gel Spot

Price: $250 per sample

Turnaround Time: 5 to 10 business days

Service Description: 

• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report

Deliverables: 

• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request

Suitable Sample Type: 

• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)

• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report

• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request

• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)

• Identification of proteins from protein gel bands or gel spots
• Up to 2,000 proteins identified from one gel band
• Workflow: Sample preparation, in-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report

• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Raw data files, upon request

• Gel band or spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (Note: additional charge applied for larger size)

+ P102: Protein Identification of Mixture Samples
Identification of Proteins (Up to 4,000 Proteins) From Any Biological Sample

Price: $500 per sample

Turnaround Time: 5 to 10 business days

Service Description:

• Identification of proteins from mixture samples including cell lysates, tissue lysates, IP, pull-down, or other types of protein mixtures
• Up to 4,000 proteins identified from one sample
• Target protein detection limit is at ng level
• Workflow: Sample preparation, In-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report

Deliverables:

• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Comparisons between and among samples using PSM# and MS1 peak area label-free based quantification method
• Raw data files, upon request

Suitable Sample type:

• Purified protein, in solution or dried
• Purified protein complex
• Protein mixture from pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches (> 1 μg is required; > 5 μg is recommended)
• Fresh frozen cell/tissue lysate, body fluids, or cell culture medium

• Identification of proteins from mixture samples including cell lysates, tissue lysates, IP, pull-down, or other types of protein mixtures
• Up to 4,000 proteins identified from one sample
• Target protein detection limit is at ng level
• Workflow: Sample preparation, In-gel enzymatic digestion, peptide extraction, LC-MS/MS analysis, data analysis, and results report

• Protein Identification Report
• List of identified proteins
• List of identified proteins with matched peptides sequences
• Comparisons between and among samples using PSM# and MS1 peak area label-free based quantification method
• Raw data files, upon request

• Purified protein, in solution or dried
• Purified protein complex
• Protein mixture from pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches (> 1 μg is required; > 5 μg is recommended)
• Fresh frozen cell/tissue lysate, body fluids, or cell culture medium

Learn more about our technology

Connect to Our Services

Contact us

Call now for immediate assistance!

301-761-4835 | [email protected]

Request Sample Reports

Sample Shipping Instructions

Please fill out the Sample Pickup Request if there is dropbox in your area

If there is no DNA-sample drop box at your location, please ship your sample to
Poochon Scientific
5350 Partners Court, Suite C,
Frederick, MD, 21703