Protein Identification and Quantification Services

The combination of High-performance Liquid Chromatography and high-resolution Tandem Mass Spectrometry (LC-MS/MS) has emerged as a powerful and universal method for the global measurement of proteins. Unlike traditional western-blot or ELISA method, MS-based technology can identify and quantify the proteins in mixture samples simultaneously at large scale. Particularly, for interested proteins without available antibody MS-based protein identification is the fastest and most reliable approach.

ID-101: Protein Identification of Gel Bands or Gel Spots

This service package is for identification of proteins (up to 500 proteins) from a gel band/spot.

Price: $250/sample; Call for a discounted quote on more than 5 samples.

Turnaround time: 5 to 10 business days.

Service Description

  • Identification of proteins from protein gel bands/spots
  • A few proteins to several hundreds of proteins can be identified from one gel band
  • Work-flow: In-gel trypsin or chymotrypsin digestion, peptide extraction, 1 hour LC-MS/MS, data analysis and result report

Deliverables

  • A list of identified proteins
  • All identified peptides sequences for each proteins
  • Method description
  • At customer request the raw data file can be sent to customer via Dropbox.

Suitable Sample Type

  • Purified protein in liquid or dried forms
  • Purified protein complex
  • Protein mixture from Pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches
  • Fresh frozen cell/tissue lysate, body fluids, or cell culture medium

Price: Contact us for a quote!

ID-102: Protein Identification of Mixture Samples

This service package is for identification of proteins (up to 2000 proteins) from any biological samples including cell lysates, tissue lysates, IP (immunoprecipitation) sample, pull-down sample, or proteins mixture.

Price: $500/sample; Call for a discounted quote on more than 5 samples.

Turnaround time: 5 to 10 business days.

 

Service Description

  • Identification of proteins from mixture samples including cell lysates, tissue lysates, IP product, pull-down product, or other type proteins mixtures;
  • Up to two thousands of proteins can be identified from one sample.
  • Work-flow: Sample preparation for MS analysis, 2-hour LC-MS/MS analysis, data analysis and Result report.

Deliverables

  • A list of identified proteins
  • All identified peptides sequences for each proteins
  • Method description
  • At customer request, the raw data file can be sent to the customer via Dropbox.

Suitable Sample Type

  • Purified protein in liquid or dried forms
  • Purified protein complex
  • Protein mixture from Pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches
  • Fresh frozen cell/tissue lysate, body fluids, or cell culture medium

Price: Contact us for a quote!