Protein Therapeutics Characterization

High-resolution characterization of therapeutic protein

Poochon developed a robust approach using a range of state-of- the-art orthogonal methodologies to elucidate the primary amino acid sequence, post-translational modifications, associated micro-heterogeneity, glycosylation associated with a therapeutic product.

HC-101: Evaluation of Identity and Purity

This service package is designed for quick evaluation of purified or partial purified protein therapeutics at its early stage of development at low cost. The readouts of this analysis include identities and approximate purity. This service package can also be used for evaluation of the purity of purified protein samples.

Price: $600/sample

Turnaround time: 10 to 15 business days.

Service Description

  • The samples will be digested by trypsin followed by LC/MS/MS analysis for identification. All detectable proteins (even at ng level) including any host proteins will be identified and their relative abundance can also be determined. The purity will be estimated based on the relative abundance of identified proteins.     
  • Work-flow:  sample preparation, trypsin or chymotrypsin digestion, LC-MS/MS analysis, data analysis and result report.

Deliverables

  • A list of identified Proteins
  • A list of identified Proteins with matched peptides sequences
  • Purity
  • Method description

Suitable Sample Type

  • Purified protein samples in liquid or dried form (> 5 µg/sample)

Price: Contact us for a quote!

HC-102: Full Amino Acid Sequence Confirmation

Confirmation of full amino acid sequence (>95% sequence coverage) of any purified protein sample (target protein amino acid sequence known) using LC/MS/MS. For de novo sequencing please contact us for a quote.

Price: $800-$1200/sample

Turnaround time: 10 to 15 business days.

Service Description

  • Confirmation of full amino acid sequence (>95% sequence coverage) of purified protein samples (purity >80%). If the sample is monoclonal antibody the cost is $1200 since both heavy chain and light chain sequences will be confirmed. All detectable proteins (even at ng level) including any host proteins will be identified and their relative abundance can be also determined. The purity will be estimated based on the relative abundance of identified proteins.    
  • Work-flow:  sample preparation, trypsin digestion and chymotrypsin digestion, LC-MS/MS analysis, data analysis and result report.

Deliverables

  • A list of Peptides identified for target protein and peptide mapping
  • A list of identified host Proteins if any
  • A list of identified host Proteins with matched peptides sequences
  • Purity
  • Method description

Suitable Sample Type

  • Purified protein samples in liquid or dried form (> 20 µg/sample, purity >80% )

Price: Contact us for a quote!

HC-103: Identification of N-linked Glycosylation sites

This service package is for identification of N-linked glycosylation site from purified protein samples (e.g. monoclonal antibody) using deglycosylation treatment and LC/MS/MS. The percentages of glycosylation will be determined using label-free quantitation method. For O-linked glycosylation please contact us for a quote.

Price: $1,800/sample

Turnaround time: 10 to 15 business days.

Service Description

  • This package is designed for identification of N-linked glycosylation sites and Confirmation of full amino acid sequence (>95% sequence coverage) of purified monoclonal antibody samples (purity >95%). All detectable proteins (even at ng level) including any host proteins will be identified and their relative abundance can be also determined. The purity will be estimated based on the relative abundance of identified proteins. This package covers HC-102.   
  • Work-flow:  sample preparation, deglycosylation treatment, trypsin digestion and chymotrypsin digestion, LC-MS/MS analysis, data analysis and result report.

Deliverables

  • N-linked glycosylation sites
  • A list of Peptides identified for target protein and peptide mapping
  • A list of identified host Proteins if any
  • A list of identified host Proteins with matched peptides sequences
  • Purity
  • Method description

Suitable Sample Type

  • Purified protein samples in liquid or dried form (> 20 µg/sample, purity >99% )
  • If the purity is less than 90% please contact us for a quote.

 

 

 

Price: Contact us for a quote!

HC-104: Profile of N-linked Glycans for mAb

This service package is for characterization of the profile of N-linked glycans released from purified monoclonal antibody samples using RapiFluor-MS™ N-Glycan labeling kit and LC/MS/MS. The relative abundance of glycans will be determined based on label-free quantitation method (Ion intensity/peak area).

Price: $5,000/sample

Turnaround time: 20 to 35 business days or less.

Service Description

  • This package is designed for profiling of N-linked glycans released form pure monoclonal antibody. This package covers the analysis of HC103. Note: If the analysis of sample identifies it has multiple N-linked glycosylation sites the N-linked glycans profiling analysis will not be preform. The cost will be the same as HC103.   
  • Work-flow:  sample preparation, deglycosylation treatment, trypsin digestion and chymotrypsin digestion, N-linked glycans labeling, LC-MS/MS analysis, data analysis and result report.

Deliverables

  • N-linked glycans profile
  • N-linked glycosylation sites
  • A list of Peptides identified for target protein and peptide mapping
  • A list of identified host Proteins if any
  • A list of identified host Proteins with matched peptides sequences
  • Purity
  • Method description

Suitable Sample Type

  • Purified protein samples in liquid or dried form (> 50 µg/sample, purity >99% )

Price: Contact us for a quote!