Other

PBS-101: LC-MS/MS – Standard 60 min LC gradient run

Analysis of peptide mixture by LC/MS/MS using QE and Ultimate 3000 nano RSLC system Sample type: Digests from in-gel or in-solution (<= 5 µg/sample)

Service Description

  1. C18 zip-tip cleanup,
  2. 60 min LC-MS/MS run,
  3. Database search,
  4. Protein ID report.

Price: Contact us for a quote!

PBS-102: LC-MS/MS – Standard 120 min LC gradient run

Analysis of peptide mixture by LC/MS/MS using QE and Ultimate 3000 nano RSLC system.
Sample type: Digests from in-gel or in-solution (<=5 µg/sample)

Service Description

  1. C18 zip-tip cleanup,
  2. 120 min LC-MS/MS run,
  3. Database search,
  4. Protein ID report

Price: Contact us for a quote!

PBS-103: Cell Lysate

Making protein lysate from cells.
Sample type: Fresh frozen cell pellet. (For profiling >1×106 cells/sample)

Service Description

  1. lyse cell,
  2. homogenization,
  3. sonication,
  4. protein content measurement using BCA kit.

Price: Contact us for a quote!

PBS-104: Tissue Lysate

Making protein lysate from tissues.
Sample type: Fresh frozen tissues. (For profiling > 100mg/sample)

Service Description

  1. lyse tissue,
  2. homogenization,
  3. sonication,
  4. protein content measurement using BCA kit.

Price: Contact us for a quote!

PBS-105: SDS-PAGE

1-9 samples

Separation of proteins on a 4-12% SDS-PAGE gel.
Sample type:

  1. Frozen liquid,
  2. in-SDS sample buffer,
  3. non-covalent on-matrix or lyophilized.

Service Description

  1. SDS-PAGE,
  2. SimplyBlue Stain,
  3. Imaging.

Price: Contact us for a quote!

PBS-106: In-gel Digestion

Trypsin or chymotrypsin digestion of proteins from gel band/spot samples.
Sample type: Gel band or gel spot, each sample should contain only one gel piece, no bigger than 1.5x2x5mm in size.

Service Description

  1. In-gel trypsin/chymotrypsin digestion,
  2. peptide extraction,
  3. C18 zip-tip cleanup

Price: Contact us for a quote!

PBS-107: In-solution Digestion

FASP (Filter Aided Sample Preparation) in-solution digest of protein mixture.
Sample type: Frozen liquid, non-covalent on-matrix or lyophilized.

Service Description

  1. In-solution digestion,
  2. peptide extraction,
  3. C18 zip-tip cleanup.

Price: Contact us for a quote!

PBS-108: TMT labeling

Preparing TMT-labeled peptides for quantitative proteome profiling. The labeling uses Thermo TMTsixplex™ or 10plex Isobaric Label Reagent and the fractionation is carried out by basic reverse phase UPLC.

Sample type: Frozen lysate, frozen cell pellet, or frozen tissue.

Service Description

  1. Protein lysate,
  2. trypsin digestion,
  3. TMT-labeling,
  4. UPLC Fractionation.

Price: Contact us for a quote!