Itemized Services

itemized services

Itemized Services

Poochon conveniently offers a variety of basic services to help complete your project. Whether you only need analysis of a peptide mixture, or simply for us to make cell lysates and measure the concentration, our itemized services will save you time and money. If a full-service package is not right for you, our itemized service options may meet your needs.

Any biological sample can be analyzed. Typical sample types include, but are not limited to: primary cells, cultured cells, PBMCs, tissues, protein mixture samples (e.g. IP and pull-down), fraction of cellular organelles (e.g. exosomes and membrane), body fluids (e.g. plasma/serum, CSF, urine, sputum, saliva, etc.), and FFPE tissue blocks/sections.

+ P1001: SDS-PAGE

Price: $100 per sample

Turnaround Time: 3 business days

Service Description: Separation of protein mixture on 4-12% NuPAGE gel

Sample Type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized

Services Include: SDS-PAGE, SimplyBlue Stain, and Imaging

Separation of protein mixture on 4-12% NuPAGE gel

Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized

SDS-PAGE, SimplyBlue Stain, and Imaging

+ P1002: In-Gel Digestion

Price: $100 per sample

Turnaround Time: 3 business days

Service Description: In-gel enzymatic digestion of proteins from gel bands/spots

Sample Type: Gel band or gel spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (additional charge applied for larger size)

Services Include: In-gel enzymatic digestion, peptide extraction, C18 zip-tip cleanup

+ P1003: In-Solution Digestion

Price: $150 per sample

Turnaround Time: 3 business days

Service Description: In-solution digestion of any biological sample

Sample Type: 3 business days

Services Include: Pierce™ BCA Protein Assay, in-solution enzymatic digestion, peptide extraction, C18 zip-tip cleanup

In-solution digestion of any biological sample

Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized

Pierce™ BCA Protein Assay, in-solution enzymatic digestion, peptide extraction, C18 zip-tip cleanup

+ P1004: LC-MS/MS (60 minute)

Price: $200 per sample

Turnaround Time: 3 business days

Service Description: Standard 60 minute gradient run

Sample Type: Digests from in-gel or in-solution (≤ 5 mg/sample)

Services Include: C18 zip-tip cleanup, 60 minute LC-MS/MS run, database search, and results report

Standard 60 minute gradient run

Digests from in-gel or in-solution (≤ 5 mg/sample)

C18 zip-tip cleanup, 60 minute LC-MS/MS run, database search, and results report

+ P1005: LC-MS/MS (110 minute)

Price: $300 per sample

Turnaround Time: 3 business days

Service Description:Standard 110 minute gradient run

Sample Type:Digests from in-gel or in-solution (≤ 5 mg/sample)

Services Include:C18 zip-tip cleanup, 110 minute LC-MS/MS run, database search, and results report

Standard 110 minute gradient run

Digests from in-gel or in-solution (≤ 5 mg/sample)

C18 zip-tip cleanup, 110 minute LC-MS/MS run, database search, and results report

+ P1006: Protein Lysate (Cell)

Price: $30 per sample

Turnaround Time: 3 business days

Service Description: Preparation of protein lysate from cell pellet

Sample Type: Fresh frozen cell pellet (profiling > 1×106 cells/sample)

Services Include: Sample preparation, sonication, and Pierce™ BCA Protein Assay

Preparation of protein lysate from cell pellet

Fresh frozen cell pellet (profiling > 1×106 cells/sample)

Sample preparation, sonication, and Pierce™ BCA Protein Assay

+ P1007: Protein Lysate (Tissue)

Price: $50 per sample

Turnaround time: 3 business days

Service Description:Preparation of protein lysate from tissue sample

Sample Type: Fresh frozen tissue (> 10 mg/sample)

Services Include: Sample preparation, homogenization, sonication, and Pierce™ BCA Protein Assay

+ P1008: TMT Labeling

Price: Quote Required

Turnaround time: 7 business days

Service Description: TMT labeling up to 18 plex using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set

Sample Type: Frozen lysate, frozen cell pellet, or frozen tissue

Services Include: Protein lysate preparation, Pierce™ BCA Protein Assay, enzymatic digestion, TMT labeling, and bRP-HPLC Fractionation

TMT labeling up to 18 plex using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set

Frozen lysate, frozen cell pellet, or frozen tissue

Protein lysate preparation, Pierce™ BCA Protein Assay, enzymatic digestion, TMT labeling, and bRP-HPLC Fractionation

+ P1009: bRP-HPLC Fractionation

Price: $1,000 per set

Turnaround time: 7 business days

Service Description:Basic reversed-phase HPLC fractionation of peptides (TMT/iTRAQ/SILAC/label-free)

Sample Type: Digested peptides mixture, labeled with TMT/iTRAQ/SILAC, or label-free

Services Include: Fractionation by basic reversed-phase HPLC using a C18 column, 150 min LC run collecting elutes into 96 wells, and combination of elutes to 24 or 12 fractions

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301-761-4835 | [email protected]

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