PBS-101: LC-MS/MS – Standard 60 min LC gradient run
Analysis of peptide mixture by LC/MS/MS using QE and Ultimate 3000 nano RSLC system Sample type: Digests from in-gel or in-solution (<= 5 µg/sample)
Service Description
- C18 zip-tip cleanup,
- 60 min LC-MS/MS run,
- Database search,
- Protein ID report.
Price: Contact us for a quote!
PBS-102: LC-MS/MS – Standard 120 min LC gradient run
Analysis of peptide mixture by LC/MS/MS using QE and Ultimate 3000 nano RSLC system.
Sample type: Digests from in-gel or in-solution (<=5 µg/sample)
Service Description
- C18 zip-tip cleanup,
- 120 min LC-MS/MS run,
- Database search,
- Protein ID report
Price: Contact us for a quote!
PBS-103: Cell Lysate
Making protein lysate from cells.
Sample type: Fresh frozen cell pellet. (For profiling >1×106 cells/sample)
Service Description
- lyse cell,
- homogenization,
- sonication,
- protein content measurement using BCA kit.
Price: Contact us for a quote!
PBS-104: Tissue Lysate
Making protein lysate from tissues.
Sample type: Fresh frozen tissues. (For profiling > 100mg/sample)
Service Description
- lyse tissue,
- homogenization,
- sonication,
- protein content measurement using BCA kit.
Price: Contact us for a quote!
PBS-105: SDS-PAGE
1-9 samples
Separation of proteins on a 4-12% SDS-PAGE gel.
Sample type:
- Frozen liquid,
- in-SDS sample buffer,
- non-covalent on-matrix or lyophilized.
Service Description
- SDS-PAGE,
- SimplyBlue Stain,
- Imaging.
Price: Contact us for a quote!
PBS-106: In-gel Digestion
Trypsin or chymotrypsin digestion of proteins from gel band/spot samples.
Sample type: Gel band or gel spot, each sample should contain only one gel piece, no bigger than 1.5x2x5mm in size.
Service Description
- In-gel trypsin/chymotrypsin digestion,
- peptide extraction,
- C18 zip-tip cleanup
Price: Contact us for a quote!
PBS-107: In-solution Digestion
FASP (Filter Aided Sample Preparation) in-solution digest of protein mixture.
Sample type: Frozen liquid, non-covalent on-matrix or lyophilized.
Service Description
- In-solution digestion,
- peptide extraction,
- C18 zip-tip cleanup.
Price: Contact us for a quote!
PBS-108: TMT labeling
Preparing TMT-labeled peptides for quantitative proteome profiling. The labeling uses Thermo TMTsixplex™ or 10plex Isobaric Label Reagent and the fractionation is carried out by basic reverse phase UPLC.
Sample type: Frozen lysate, frozen cell pellet, or frozen tissue.
Service Description
- Protein lysate,
- trypsin digestion,
- TMT-labeling,
- UPLC Fractionation.