Itemized Services
Itemized Services
Poochon conveniently offers a variety of basic services to help complete your project. Whether you only need analysis of a peptide mixture, or simply for us to make cell lysates and measure the concentration, our itemized services will save you time and money. If a full-service package is not right for you, our itemized service options may meet your needs.
Any biological sample can be analyzed. Typical sample types include, but are not limited to: primary cells, cultured cells, PBMCs, tissues, protein mixture samples (e.g. IP and pull-down), fraction of cellular organelles (e.g. exosomes and membrane), body fluids (e.g. plasma/serum, CSF, urine, sputum, saliva, etc.), and FFPE tissue blocks/sections.
+ P1001: SDS-PAGE
Turnaround Time: 3 business days
Service Description: Separation of protein mixture on 4-12% NuPAGE gel
Sample Type: Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
Services Include: SDS-PAGE, SimplyBlue Stain, and Imaging
Separation of protein mixture on 4-12% NuPAGE gel
Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
SDS-PAGE, SimplyBlue Stain, and Imaging
+ P1002: In-Gel Digestion
Turnaround Time: 3 business days
Service Description: In-gel enzymatic digestion of proteins from gel bands/spots
Sample Type: Gel band or gel spot, no larger than 3mm (l) x 3.5mm (h) x 1.5mm (w) in size (additional charge applied for larger size)
Services Include: In-gel enzymatic digestion, peptide extraction, C18 zip-tip cleanup
+ P1003: In-Solution Digestion
Turnaround Time: 3 business days
Service Description: In-solution digestion of any biological sample
Sample Type: 3 business days
Services Include: Pierce™ BCA Protein Assay, in-solution enzymatic digestion, peptide extraction, C18 zip-tip cleanup
In-solution digestion of any biological sample
Frozen liquid, in-SDS sample buffer, non-covalent on-matrix or lyophilized
Pierce™ BCA Protein Assay, in-solution enzymatic digestion, peptide extraction, C18 zip-tip cleanup
+ P1004: LC-MS/MS (60 minute)
Turnaround Time: 3 business days
Service Description: Standard 60 minute gradient run
Sample Type: Digests from in-gel or in-solution (≤ 5 mg/sample)
Services Include: C18 zip-tip cleanup, 60 minute LC-MS/MS run, database search, and results report
Standard 60 minute gradient run
Digests from in-gel or in-solution (≤ 5 mg/sample)
C18 zip-tip cleanup, 60 minute LC-MS/MS run, database search, and results report
+ P1005: LC-MS/MS (110 minute)
Turnaround Time: 3 business days
Service Description: Standard 110 minute gradient run
Sample Type: Digests from in-gel or in-solution (≤ 5 mg/sample)
Services Include: C18 zip-tip cleanup, 110 minute LC-MS/MS run, database search, and results report
Standard 110 minute gradient run
Digests from in-gel or in-solution (≤ 5 mg/sample)
C18 zip-tip cleanup, 110 minute LC-MS/MS run, database search, and results report
+ P1006: Protein Lysate (Cell)
Turnaround Time: 3 business days
Service Description: Preparation of protein lysate from cell pellet
Sample Type: Fresh frozen cell pellet (profiling > 1×106 cells/sample)
Services Include: Sample preparation, sonication, and Pierce™ BCA Protein Assay
Preparation of protein lysate from cell pellet
Fresh frozen cell pellet (profiling > 1×106 cells/sample)
Sample preparation, sonication, and Pierce™ BCA Protein Assay
+ P1007: Protein Lysate (Tissue)
Turnaround time: 3 business days
Service Description: Preparation of protein lysate from tissue sample
Sample Type: Fresh frozen tissue (> 10 mg/sample)
Services Include: Sample preparation, homogenization, sonication, and Pierce™ BCA Protein Assay
+ P1008: TMT Labeling
Turnaround time: 7 business days
Service Description: TMT labeling up to 18 plex using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set
Sample Type: Frozen lysate, frozen cell pellet, or frozen tissue
Services Include: Protein lysate preparation, Pierce™ BCA Protein Assay, enzymatic digestion, TMT labeling, and bRP-HPLC Fractionation
TMT labeling up to 18 plex using Thermo Scientific TMTpro™ 18-Plex Label Reagent Set
Frozen lysate, frozen cell pellet, or frozen tissue
Protein lysate preparation, Pierce™ BCA Protein Assay, enzymatic digestion, TMT labeling, and bRP-HPLC Fractionation
+ P1009: bRP-HPLC Fractionation
Turnaround time: 7 business days
Service Description:Basic reversed-phase HPLC fractionation of peptides (TMT/iTRAQ/SILAC/label-free)
Sample Type: Digested peptides mixture, labeled with TMT/iTRAQ/SILAC, or label-free
Services Include: Fractionation by basic reversed-phase HPLC using a C18 column, 150 min LC run collecting elutes into 96 wells, and combination of elutes to 24 or 12 fractions
