Post-Translational Modifications Analysis

Proteins are subjected to a wide variety of covalent modifications after translation. Many of these post-translational modifications (PTMs) are critical to the protein's function. The common PTMs includes Phosphorylation, Ubiquitination, Methylation, Acetylation and Glycosylation. We have accumulated experiences and developed reliable workflows using available tools and technologies for identifications of PTMs.

PTM-101: PTM Identification

This service package is for identification of PTMs including S/T/Y phosphorylation, R/K methylation, K/H acetylation, K ubiquitination, etc. from crude or partial purified or purified protein samples.

Price: $600/sample

Turnaround time: 10 to 15 business days or less.

Service Description

  • Identification of PTMs (S/T/Y phosphorylation, R/K methylation, K/H acetylation, K ubiquitination sites for a target protein from purified protein samples or partial purified protein mixture samples. For identification of specific PTMs, please contact us for a quote.
  • Work-flow: sample preparation, LC-MS/MS analysis, Data analysis and result report.

Deliverables

  • A list of identified proteins
  • All identified peptides sequences with PTMs for target protein
  • PTM’s spectra
  • Method description

Suitable Sample Type

  • Purified protein in liquid or dried forms
  • Purified protein complex
  • Protein mixture from Pull-down, immunoprecipitation (IP), co-immunoprecipitation (co-IP), or other approaches
  • Certain amount of target protein(s) is required, e.g. 0.5 µg of a target protein

Price: Contact us for a quote!

PTM-102: Phosphoprotein Profiling

This service package is for profiling of S/T/Y phosphorylation sites using phosphopeptides enrichment approach and label-free quantification method for comparison among different samples.

Price: $2000/sample

Turnaround time: 15 to 20 business days or less.

Service Description

  • Profiling of S/T/Y phosphorylation sites at global level from treated cell pellets (> 2×107 cell/sample), fresh frozen tissues (>500 mg/sample), or fresh frozen total cell or tissue lysates (200 µl at 5 mg/ml). For profiling of specific PTMs, please contact us for a quote.
  • Normally, up to one thousand of phosphorylation sites can be identified from an analysis. This service can be tailored for quantitatively profiling and comparing between two samples or between one control and several different treatments.
  • Work-flow: sample preparation, phosphopeptides enrichment using TiO2 beads, LC-MS/MS analysis, Data analysis and result report.

Deliverables

  • A list of identified proteins
  • All identified peptides sequences with phosphorylation sites
  • Label-free quantification
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥20 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (≥500 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥500 μg of total protein recommended for optimal results)

Price: Contact us for a quote!

PTM-103: TMTsixplex-labeling Quantitative Phosphoprotein profiling

This service package quantitatively compare the Phospho-proteomes of 2-6 samples in one set of analyses using Thermo TMTsixplex™ Isobaric Label Reagent and Phosphopeptides enrichment kit.

Price: $9,000-$12,000/set.

Turnaround time: 20 to 25 business days.

Service Description

  • Quantitative profiling of phosphoproteins at a global level for up to six samples in one set of analyses using Thermo TMTsixplex™ Isobaric Label Reagent and Phosphopeptides enrichment kit. Recommend for analysis of two groups of samples in triplicates each group.    
  • Approximate 1000 phosphorylation sites can be identified at 1% FDR.
  • Pathway analysis of identified differential phosphoproteins using PROMICPATH.
  • Work-flow:  Protein lysate preparation, TMT-labeling, UHPLC fractionation, TiO2 Phosphopeptides enrichment, LC-MS/MS analysis, TMT quantitation, Pathway analysis, and result report.

Deliverables

  • A list of identified Phospho-proteins with TMT-tag based relative abundance
  • A list of all identified Phospho-peptides sequences with phosphorylation sites
  • A list of phospho-proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥20 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (≥500 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥500 μg of total protein per sample is recommended for optimal results)

Price: Contact us for a quote!

PTM-104: TMT10plex-labeling Quantitative Phosphoprotein Profiling

This service package quantitatively compare the Phospho-proteomes of 7-10 samples in one set of analysis using Thermo TMT10plex™ Isobaric Label Reagent and Phosphopeptides enrichment kit.

Price: $12,500-$16,000/set

Turnaround time: 20 to 25 business days.

Service Description

  • Quantitative profiling of phosphoproteins at global level for up to ten samples in one set of analyses using Thermo TMTsixplex™ Isobaric Label Reagent and Phosphopeptides enrichment kit. Recommend for analysis of three groups of samples in triplicates each group.    
  • Approximate 1000 phosphorylation sites can be identified at 1% FDR.
  • Pathway analysis of identified differential phosphoproteins using PROMICPATH.
  • Work-flow:  Protein lysate preparation, TMT-labeling, UHPLC fractionation, TiO2 Phosphopeptides enrichment, LC-MS/MS analysis, TMT quantitation, Pathway analysis, and result report.

Deliverables

  • A list of identified Phospho-proteins with TMT-tag based relative abundance
  • A list of all identified Phospho-peptides sequences with phosphorylation sites
  • A list of phospho-proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥20 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (≥500 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥500 μg of total protein per sample is recommended for optimal results)

Price: Contact us for a quote!