Peptide Mapping
+ P501: Peptide Mapping
Protein Sequence Confirmation of a Test Protein Product Compared to a Reference Standard
Turnaround Time: 5 to 10 business days
Service Description:
• Protein sequence confirmation and determination of post-translational modifications (PTMs) and sequence variants by comparison to a reference standard or predicted sequence (e.g., used when comparing biosimilars with versions of original “innovator” products)
• Can be used for proof of identity, primary structural characterization, quality assurance (QA), and characterization of a new reference standard
• Workflow: Sample preparation, protease(s) selection, enzymatic digestion, LC-MS/MS analysis, data analysis, and results report
Deliverables
• Protein analysis report
• Peptide mapping results (alignment of digested peptides to predicted sequence)
• List of peptides detected
• Chromatograms of enzymatic digests of sample and comparison with reference standard if any
• Raw data files, upon request
Suitable Sample Type
• Purified protein sample, in solution or dried (≥ 20 µg per sample recommended for optimal results; purity > 99%)
• Protein sequence confirmation and determination of post-translational modifications (PTMs) and sequence variants by comparison to a reference standard or predicted sequence (e.g., used when comparing biosimilars with versions of original “innovator” products)
• Can be used for proof of identity, primary structural characterization, quality assurance (QA), and characterization of a new reference standard
• Workflow: Sample preparation, protease(s) selection, enzymatic digestion, LC-MS/MS analysis, data analysis, and results report
• Protein analysis report
• Peptide mapping results (alignment of digested peptides to predicted sequence)
• List of peptides detected
• Chromatograms of enzymatic digests of sample and comparison with reference standard if any
• Raw data files, upon request
• Purified protein sample, in solution or dried (≥ 20 µg per sample recommended for optimal results; purity > 99%)