Proteomic Profiling and Quantification Services

Proteins are the key functional actors in all cellular processes; diseases almost always manifest at the level of proteins. The analysis of all expressed proteins (the proteome), for example healthy and diseased tissues, is definitely the most applicable approach to uncover key players, which are often named as biomarkers or functionally related protein signatures. Poochon’s PROMIC platform allows for deep quantitative protein profiling up to 7,000 Proteins in one single experiment, and up to 15,000 proteins for one project. Label-free quantification, SILAC labeling based quantification, or TMT isobaric multiplex (six or ten plex) labeling quantification is available. Additionally, the data generated by PROMIC platform can be analyzed using Poochon’s PROMICPATH pathway analysis tool, which uses ten groups of housekeeping proteins/complexes to evaluate the completeness and variability of a proteome profile. The evaluation ensures the integrity, reliability and reproducibility of the large set of protein profile data generated by PROMIC platform.

QT-101: Label-free Quantitative Proteome Profiling

This service package is for proteome profiling (identify up to 5,000 proteins per sample) from human/mouse tissue or cell line samples using label-free quantitation method. This service package is also for proteome profiling of samples from other species. Please contact us for a quote.

Price: $3,000/sample

Turnaround time: 15 to 20 business days

Service Description

  • Identification and quantification of the entire set of proteins (the proteome) expressed by a cell, tissue or organism as many as possible.
  • More than 4000 (QT-101) proteins can be identified at 1% FDR from one sample.
  • The profile is evaluated for integrity and variability using PROMICPATH.
  • Work-flow: Protein lysate preparation, fractionation (16 fractions), digestion, 16 LC-MS/MS 110 min runs, common pathway analysis, and result report.

Deliverables

  • A list of identified proteins with relative abundance
  • A list of all identified proteins with matched peptides sequences
  • A list of proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥ 2 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (200 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥200 μg of total protein recommended for optimal results)

Price: Contact us for a quote!

QT-102: Label-free Quantitative Proteome Profiling

This service package is for proteome profiling (identify up to 7,000 proteins per sample) from human/mouse tissue or cell line samples using label-free quantitation method.

Price: $4,200/sample

Turnaround time: 15 to 20 business days.

Service Description

  • Identification and quantification of the entire set of proteins (the proteome) expressed by a cell, tissue or organism as many as possible.
  • More than 6000 (QT-102) proteins can be identified at 1% FDR from one sample.
  • The profile is evaluated for integrity and variability using PROMICPATH.
  • Work-flow: Protein lysate preparation, fractionation (24 fractions), digestion, 24 LC-MS/MS 110 min runs, common pathway analysis, and result report.

Deliverables

  • A list of identified proteins with relative abundance
  • A list of all identified proteins with matched peptides sequences
  • A list of proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥ 2 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (200 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥200 μg of total protein recommended for optimal results)

Price: Contact us for a quote!

QT-201: TMTsixplex-Labeling Quantitative Proteome Profiling

This service package is for quantitatively comparison of the proteomes (>5000 proteins if human or mouse origin samples) of 2-6 samples in one set of analysis using Thermo TMTsixplex™ Isobaric Label Reagent.

Price: $8,000-$10,000/set

Turnaround time: 20 to 25 business days.

Service Description

  • Identification and quantification of the entire set of proteins (the proteome) expressed by a cell, tissue or organism for up to 10 samples in one set of analysis simultaneously.
  • More than 5000 proteins can be identified at 1% FDR from one sample.
  • The profile is evaluated for integrity and variability using PROMICPATH.
  • Work-flow:  Protein lysate preparation, TMT-labeling, UHPLC fractionation, digestion, LC-MS/MS analysis, TMT quantitation, Pathway analysis, and result report.

Deliverables

  • A list of identified proteins with TMT-tag based relative abundance
  • A list of all identified proteins with matched peptides sequences
  • A list of proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥10 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (≥500 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥500 μg of total protein recommended for optimal results)

Price: Contact us for a quote!

QT-202: TMT10plex-Labeling Quantitative Proteome Profiling

This service package is for quantitatively comparison of the proteomes (>5000 proteins if human or mouse origin samples) of 7-10 samples in one set of analysis using Thermo TMT10plex™ Isobaric Label Reagent.

Price: $12,000-$15,000/set

Turnaround time: 20 to 25 business days.

Service Description

  • Identification and quantification of the entire set of proteins (the proteome) expressed by a cell, tissue or organism for up to 10 samples in one set of analysis simultaneously.
  • More than 5000 proteins can be identified at 1% FDR.
  • The profile is evaluated for integrity and variability using PROMICPATH.
  • Work-flow:  Protein lysate preparation, TMT10plex-labeling, UHPLC fractionation, digestion, LC-MS/MS analysis, TMT quantitation, Pathway analysis, and result report.

Deliverables

  • A list of identified proteins with TMT-tag based relative abundance
  • A list of all identified proteins with matched peptides sequences
  • A list of proteins which is differentially expressed among samples (e.g. >2 folds)
  • Common pathway analysis results
  • Method description

Suitable Sample Type

  • Fresh frozen cell pellet (≥10 million cells per sample recommended for optimal results)
  • Fresh frozen tissue (≥500 mg in weight recommended for optimal results)
  • Total Cell or tissue lysate (≥500 μg of total protein recommended for optimal results)

 

Price: Contact us for a quote!